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Knowledgebase: Synbiosis
ProtoCOL 2 Count FAQ
Posted by Keira Davies, Last modified by Keira Davies on 28 July 2016 04:52 PM

Please note: the ProtoCOL 2 is a discontinued product.  Please do not assume that the information below applies to our more recent ProtoCOL systems.

What is the smallest colony detectable by the ProtoCOL 2 colony counter?

ProtoCOL 2 colony counter uses a CCD camera to capture a high definition image and detects colonies as small as 43 microns.

Can the ProtoCOL 2 colony counter be connected to a LIMS system?

The ProtoCOL 2 colony counter can be connected to a personal computer, or LIMS system, using the serial port contained on the ProtoCOL system. Four data formats are available, from simple plate Id and count per ml, to every item of data stored for each plate.

Can the ProtoCOL 2 count colonies on selective agars?

Yes, the ProtoCOL 2 colony counter can count colonies on any type of agar. The ProtoCOL 2 has an innovative lighting system which exposes the plate to rapid sequential bursts of red, green and blue light. The resulting colour composite image has excellent definition and clarity with no chromatic aberrations (patent pending). An automated self calibration process ensures accurate colour definition with each exposure.

Will the ProtoCOL 2 colony counter transfer data into other software packages?

The ProtoCOL 2 has the ability to transfer data, on request, into Microsoft Excel (optional extra). In addition, the ProtoCOL 2 can save the results to a text file, or comma separated value (csv) file, which provides wide flexibility with other software packages.

How does the ProtoCOL 2 colony counter count spiral plates?

When a two sector spiral plate is counted, the procedure is as follows:

Colonies in the outer sector of the upper half of the spiral are counted. If this count is less than the minimum count rule value (usually 20), then the count of the next sector towards the centre is also counted. This procedure of counting an additional sector towards the centre is continued until the minimum count value is exceeded. When the count is exceeded, the equivalent number of sectors is counted on the lower half of the spiral. These two values are added together and displayed as the count per frame. The count per ml is calculated by dividing the count per frame by the entered sector value for the spiral plater model used. If the count in the upper half of the spiral doesn’t reach 20 when all the sectors have been counted, the ProtoCOL 2 will automatically count the whole plate, ignoring the count rule.

Can ProtoCOL 2 count spirals on the new 85mm diameter Oxoid plates?

Yes, the plate size is user selectable in the software and the correct count tables are automatically selected.

How do you connect a barcode to the ProtoCOL 2 and what can you do with it?

A barcode reader can be connected to the ProtoCOL 2 using one of the four free USB ports. The barcode is used to enter the plate ID, and dilution, if required before it is measured.

Can a comment be added to a batch and displayed when it is selected?

Yes, when a new batch is created a textual comment can be entered at anytime, and the comment will be displayed within the report.

Is ProtoCOL 2 CFR compliant?

Yes, the system meets all 21 CFR part 11 requirements.

Does ProtoCOL 2 provide an audit trail?

A full audit trail is available within the ProtoCOL 2 software. This can be details of a change made to a particular batch, or a complete audit log of every batch on the ProtoCOL 2 unit.

How is data stored on ProtoCOL 2?

All measurement data is automatically saved to a Microsoft SQL Server database. Images are saved to file, but can be stored in the database if the system is upgraded to use a remote database server.

Can ProtoCOL 2 be used to count fluorescing colonies?

Yes, the Proc UV system can be used to count and distinguish between fluorescing and non fluorescing colonies.

What is the largest plate size that ProtoCOL 2 can image?

100mm is currently the largest diameter of plate the ProtoCOL 2 can measure, but larger plates can be imaged using the macrostand provided in the ProcMacro system. This allows plates up to 150mm to be imaged and analysed using ProtoCOL 2 software.

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